Cell-Free Synthetic Biology
Material type:
ArticlePublication details: MDPI - Multidisciplinary Digital Publishing Institute 2020Description: 1 electronic resource (152 p.)Content type: - text
- computer
- online resource
- 9783039280223
- 9783039280230
- Technology, Engineering, Agriculture, Industrial processes
- Technology: general issues
- History of engineering and technology
- CFPS
- DNA origami
- E. coli crude extract preparation
- antimicrobials
- artificial cell
- bioconversion platform
- cell-free metabolic engineering
- cell-free metabolic engineering (CFME)
- cell-free protein expression (CFPE)
- cell-free protein synthesis
- cell-free protein synthesis (CFPS)
- cell-free synthetic biology
- cell-free transcription-translation
- chaperones
- colicins
- drug development
- enzymes
- eukaryotic lysates
- genomically engineered E. coli
- growth factors
- in vitro protein synthesis
- in vitro transcription-translation (TX-TL)
- in vitro translation
- ischemic stroke
- mathematical model
- microsomes
- protein production
- rapid prototyping
- riboregulator
- sonication
- synthetic biology
- tPa
- tissue plasminogen activator
- unnatural amino acid
- unnatural protein
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Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas.
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eng
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