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The growing demand for high-throughput separations in food, environmental, clinical, and bioanalytical research has increased the need for methods capable of efficiently analyzing complex matrices with both qualitative and quantitative precision. High-performance liquid chromatography (HPLC) is a well-established separation technique widely employed in many fields. Its versatility of chromatographic separation modes (reversed-phase, normal-phase, HILIC, ion-chromatography, multidimensional-chromatography), chromatographic column technologies (conventional HPLC columns, sub-2 µm UHPLC columns, or partially porous core-shell columns), and detection systems (ultraviolet-visible, fluorescence, amperometric), as well as its coupling with low-resolution and high-resolution mass spectrometry, makes HPLC among the best options to solve emerging analytical problems. This book provides a comprehensive overview of new advances and applications of HPLC in environmental, food, clinical, and bioanalytical fields.

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