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The second edition of this Special Issue, entitled "Antimicrobial and Anti-Infective Activity of Natural Products", contains 12 research and 2 review articles. They present recent advancements in the discovery of bioactive compounds from plants, essential oils, and microbial metabolites. These contributions discuss the mechanisms of action, highlighting anti-virulence strategies, biofilm inhibition, and immunomodulatory effects. Furthermore, this collection explores synergistic associations with conventional antibiotics and integrates in silico, in vitro, and in vivo evidence. This volume provides critical insights into the clinical prospects of natural products in combating the growing threat of antimicrobial resistance.

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